产品描述

This Human IKZF3 ELISA Kit was designed for the quantitative measurement of Human IKZF3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

AIO ELISA Kit; Aiolos ELISA Kit; IKAROS family zinc finger 3 (Aiolos) ELISA Kit; IKAROS family zinc finger 3 ELISA Kit; Ikaros family zinc finger protein 3 ELISA Kit; IKZF 3 ELISA Kit; IKZF3 ELISA Kit; IKZF3_HUMAN ELISA Kit; zinc finger DNA binding protein Aiolos ELISA Kit; Zinc finger protein Aiolos ELISA Kit; Zinc finger protein subfamily 1A 3 (Aiolos) ELISA Kit; Zinc finger protein subfamily 1A 3 ELISA Kit; Zinc finger protein subfamily 1A; member 3 ELISA Kit; ZNFN1A3 ELISA Kit

缩写

IKZF3

Uniprot No

Q9UKT9

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IKZF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-91
1:2		Average %		96
		Range %		91-104
1:4		Average %		96
		Range %		92-109
1:8		Average %		94
		Range %		86-97

回收率

The recovery of human IKZF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		96		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.098 1.986 2.042 1.950 800 1.357 1.298 1.328 1.236 400 0.825 0.789 0.807 0.715 200 0.500 0.481 0.491 0.399 100 0.360 0.351 0.356 0.264 50 0.245 0.240 0.243 0.151 25 0.156 0.152 0.154 0.062 0 0.092 0.091 0.092

货期

3-5 working days