产品描述
This Pig IL12B ELISA Kit was designed for the quantitative measurement of Pig IL12B protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
IL12BInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写
IL12B
Uniprot No
Q28938
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 80-99 | |
| 1:2 | Average % | 95 | |
| Range % | 90-104 | |
| 1:4 | Average % | 98 | |
| Range % | 92-110 | |
| 1:8 | Average % | 95 | |
| Range % | 85-105 | |
回收率
| The recovery of pig IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 85-104 | |
| EDTA plasma (n=4) | 98 | 90-112 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.028 | 2.157 | 2.093 | 1.884 | | | 500 | 1.562 | 1.612 | 1.587 | 1.378 | | | 250 | 0.980 | 1.034 | 1.007 | 0.798 | | | 125 | 0.570 | 0.603 | 0.587 | 0.378 | | | 62.5 | 0.352 | 0.362 | 0.357 | 0.148 | | | 31.25 | 0.303 | 0.311 | 0.307 | 0.098 | | | 15.6 | 0.282 | 0.268 | 0.275 | 0.066 | | | 0 | 0.214 | 0.204 | 0.209 | | |
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货期
3-5 working days