产品描述
This Rabbit PDGF-AB ELISA Kit was designed for the quantitative measurement of Rabbit PDGF-AB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
缩写
PDGF-AB
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-92 | |
| 1:2 | Average % | 87 | |
| Range % | 83-95 | |
| 1:4 | Average % | 94 | |
| Range % | 90-98 | |
| 1:8 | Average % | 101 | |
| Range % | 96-105 | |
回收率
| The recovery of rabbit PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 83-93 | |
| EDTA plasma (n=4) | 93 | 89-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.846 | 2.734 | 2.790 | 2.696 | | | 50 | 2.147 | 2.035 | 2.091 | 1.997 | | | 25 | 1.293 | 1.248 | 1.271 | 1.177 | | | 12.5 | 0.688 | 0.677 | 0.683 | 0.589 | | | 6.25 | 0.412 | 0.424 | 0.418 | 0.324 | | | 3.12 | 0.278 | 0.289 | 0.284 | 0.190 | | | 1.56 | 0.151 | 0.153 | 0.152 | 0.058 | | | 0 | 0.093 | 0.094 | 0.094 | | |
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货期
3-5 working days