产品描述
This Rat IL-35 ELISA Kit was designed for the quantitative measurement of Rat IL-35 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
IL-35
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 92 | | |
| Range % | 88-93 | | |
| 1:2 | Average % | 94 | | |
| Range % | 90-102 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:8 | Average % | 95 | | |
| Range % | 91-98 | | |
回收率
| The recovery of rat IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 88-96 | | |
| EDTA plasma (n=4) | 98 | 93-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 400 | 2.201 | 2.321 | 2.261 | 2.068 | | | | 200 | 1.678 | 1.750 | 1.714 | 1.521 | | | | 100 | 1.143 | 1.165 | 1.154 | 0.961 | | | | 50 | 0.746 | 0.748 | 0.747 | 0.554 | | | | 25 | 0.439 | 0.468 | 0.454 | 0.261 | | | | 12.5 | 0.298 | 0.302 | 0.300 | 0.107 | | | | 6.25 | 0.245 | 0.255 | 0.250 | 0.057 | | | | 0 | 0.188 | 0.198 | 0.193 | | | |
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货期
3-5 working days