产品描述
This Rat IL11 ELISA Kit was designed for the quantitative measurement of Rat IL11 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
Il11 ELISA Kit; Interleukin-11 ELISA Kit; IL-11 ELISA Kit
缩写
IL11
Uniprot No
Q99MF5
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 81-94 | |
| 1:2 | Average % | 85 | |
| Range % | 80-90 | |
| 1:4 | Average % | 92 | |
| Range % | 86-101 | |
| 1:8 | Average % | 95 | |
| Range % | 89-102 | |
回收率
| The recovery of rat IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 80-105 | |
| EDTA plasma (n=4) | 98 | 90-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.179 | 2.116 | 2.148 | 2.055 | | | 500 | 1.773 | 1.704 | 1.739 | 1.646 | | | 250 | 1.426 | 1.313 | 1.370 | 1.277 | | | 125 | 1.009 | 0.959 | 0.984 | 0.891 | | | 62.5 | 0.537 | 0.517 | 0.527 | 0.434 | | | 31.2 | 0.262 | 0.250 | 0.256 | 0.163 | | | 15.6 | 0.198 | 0.186 | 0.192 | 0.099 | | | 0 | 0.095 | 0.090 | 0.093 | | |
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货期
3-5 working days