产品描述
This Mouse D-LDH ELISA Kit was designed for the quantitative measurement of Mouse D-LDH protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 mU/mL-300 mU/mL and the sensitivity is 1.17 mU/mL.
别名
Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
缩写
LDHD
Uniprot No
Q7TNG8
灵敏度
1.17 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:2 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:4 |
Average % |
87 |
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| Range % |
83-90 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-102 |
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回收率
| The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-95 |
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| EDTA plasma (n=4) |
104 |
101-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.278 |
2.304 |
2.291 |
2.111 |
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| 150 |
1.422 |
1.500 |
1.461 |
1.281 |
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| 75 |
0.932 |
0.921 |
0.927 |
0.747 |
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| 37.5 |
0.623 |
0.624 |
0.624 |
0.444 |
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| 18.75 |
0.385 |
0.394 |
0.390 |
0.210 |
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| 9.38 |
0.287 |
0.275 |
0.281 |
0.101 |
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| 4.69 |
0.242 |
0.231 |
0.237 |
0.057 |
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| 0 |
0.181 |
0.179 |
0.180 |
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货期
3-5 working days