产品描述
This Rat NOS1 ELISA Kit was designed for the quantitative measurement of Rat NOS1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 mIU/mL-2000 mIU/mL and the sensitivity is 7.81 mLU/mL .
别名
Nos1 ELISA Kit; BnosNitric oxide synthase ELISA Kit; brain ELISA Kit; EC 1.14.13.39 ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; NC-NOS ELISA Kit; NOS type I ELISA Kit; Neuronal NOS ELISA Kit; N-NOS ELISA Kit; nNOS ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit
缩写
NOS1
Uniprot No
P29476
灵敏度
7.81 mLU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat nNOS/NOS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 93 | |
| Range % | 87-97 | |
| 1:10 | Average % | 97 | |
| Range % | 92-103 | |
| 1:20 | Average % | 95 | |
| Range % | 91-99 | |
| 1:40 | Average % | 95 | |
| Range % | 89-101 | |
回收率
| The recovery of rat nNOS/NOS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 85-94 | |
| EDTA plasma (n=4) | 92 | 89-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mlU/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.159 | 2.256 | 2.208 | 2.055 | | | 1000 | 1.587 | 1.609 | 1.598 | 1.445 | | | 500 | 1.213 | 1.156 | 1.185 | 1.032 | | | 250 | 0.844 | 0.850 | 0.847 | 0.694 | | | 125 | 0.489 | 0.465 | 0.477 | 0.324 | | | 62.5 | 0.285 | 0.277 | 0.281 | 0.128 | | | 31.25 | 0.235 | 0.253 | 0.244 | 0.091 | | | 0 | 0.156 | 0.150 | 0.153 | | |
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货期
3-5 working days