产品描述
The Human Glycodelin ELISA Kit is a reliable tool for quantitative detection of Glycodelin in various sample types. Glycodelin, also known as Pregnancy-Associated Endometrial alpha-2-globulin (PAEP), is a glycoprotein that plays a crucial role in reproduction and immunomodulation.
This ELISA kit is designed for the detection of human Glycodelin in serum, cerebrospinal fluid (CSF), urine, and tissue homogenates. Based on a sandwich assay principle, this kit provides accurate and reproducible results with minimal interference from other proteins.
With a sensitivity of 0.195 ng/mL and a detection range of 0.78 ng/mL-50 ng/mL, this ELISA kit can accurately measure Glycodelin levels in various sample types. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of Glycodelin levels.
This Glycodelin ELISA Kit has been cited in more than 2 publications, demonstrating its effectiveness and reliability in research studies. The ELISA kit is an essential tool for researchers studying the role of Glycodelin in reproduction, immunomodulation, and various diseases such as cancer.
别名
Alpha uterine protein ELISA Kit; gD ELISA Kit; GdA ELISA Kit; GdF ELISA Kit; GdS ELISA Kit; Glycodelin A ELISA Kit; Glycodelin ELISA Kit; Glycodelin F ELISA Kit; Glycodelin S ELISA Kit; MGC138509 ELISA Kit; MGC142288 ELISA Kit; PAEG ELISA Kit; PAEP ELISA Kit; PAEP_HUMAN ELISA Kit; PEG ELISA Kit; PEP ELISA Kit; Placental Protein 14 / Glycodelin A ELISA Kit; Placental protein 14 ELISA Kit; PP14 ELISA Kit; Pregnancy-associated endometrial alpha-2 globulin ELISA Kit; Progestagen-associated endometrial protein ELISA Kit; Progesterone-associated endometrial protein ELISA Kit
缩写
PAEP
Uniprot No
P09466
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human glycodelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-92 | |
| 1:2 | Average % | 99 | |
| Range % | 94-105 | |
| 1:4 | Average % | 103 | |
| Range % | 92-110 | |
| 1:8 | Average % | 91 | |
| Range % | 86-96 | |
回收率
| The recovery of human glycodelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 115 | 110-120 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.821 | 2.687 | 2.754 | 2.631 | | | 25 | 2.287 | 2.193 | 2.240 | 2.117 | | | 12.5 | 1.642 | 1.574 | 1.608 | 1.485 | | | 6.25 | 0.964 | 0.931 | 0.948 | 0.825 | | | 3.12 | 0.581 | 0.563 | 0.572 | 0.449 | | | 1.56 | 0.389 | 0.382 | 0.386 | 0.263 | | | 0.78 | 0.265 | 0.257 | 0.261 | 0.138 | | | 0 | 0.125 | 0.121 | 0.123 | | |
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货期
3-5 working days