产品描述

CUSABIO’s Neurogranin(NRGN) ELISA kit is specifically designed and validated for the quantitative detection of NRGN in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for neuroscience research use in humans. The kit has been validated with excellent sensitivity, high specificity, precision less than 10%, good linearity, high recovery, and tiny inter-batch differences. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which NRGN in the samples or standards are sandwiched between pre-coated NRGN antibody and Biotin-conjugated NRGN antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of NRGN bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of NRGN in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

NRGN is a small neuronal protein present mainly in the cell body and dendrites. It primarily seems to regulate calmodulin (CaM) signal transduction pathways of CaM-dependent enzymes to enhance synaptic plasticity in long-term potentiation (LTP). Keeping NRGN concentrations in a normal range in neurons is essential for proper cognition, while a reduction of NRGN upon aging and degenerative diseases may lead to negative cognitive symptoms. Increased levels of NRGN has been detected in the cerebrospinal fluid (CSF) of patients with Alzheimer's disease (AD).

别名

缩写

Uniprot No

反应时间

样本体积

检测波长

研究领域

测定原理

货期