产品描述

This Human MMP10 ELISA Kit was designed for the quantitative measurement of Human MMP10 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

Matrix metallopeptidase 10 (stromelysin 2) ELISA Kit; Matrix metalloprotease 10 ELISA Kit; Matrix metalloproteinase 10 (stromelysin 2) ELISA Kit; Matrix metalloproteinase 10 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP 10 ELISA Kit; MMP-10 ELISA Kit; Mmp10 ELISA Kit; MMP10_HUMAN ELISA Kit; SL 2 ELISA Kit; SL-2 ELISA Kit; SL2 ELISA Kit; STMY 2 ELISA Kit; STMY2 ELISA Kit; Stromelysin 2 ELISA Kit; Stromelysin II ELISA Kit; Stromelysin-2 ELISA Kit; Stromelysin2 ELISA Kit; StromelysinII ELISA Kit; Transin 2 ELISA Kit; Transin-2 ELISA Kit; Transin2 ELISA Kit

缩写

MMP10

Uniprot No

P09238

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-102
1:2		Average %		107
		Range %		102-110
1:4		Average %		95
		Range %		90-101
1:8		Average %		89
		Range %		86-96

回收率

The recovery of human MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		99-105
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 5000 1.899 1.877 1.888 1.779 2500 1.354 1.366 1.360 1.251 1250 0.924 0.966 0.945 0.836 625 0.622 0.654 0.638 0.529 312 0.433 0.455 0.444 0.335 156 0.270 0.278 0.274 0.165 78 0.238 0.233 0.236 0.127 0 0.112 0.105 0.109 　 .

货期

3-5 working days