产品描述
This Rat NFκB p65 ELISA Kit was designed for the quantitative measurement of Rat NFκB p65 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
NF-Kappa B,nuclear factor-kappa B
缩写
NFκB p65
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 89 | |
| Range % | 86-92 | |
| 1:200 | Average % | 99 | |
| Range % | 97-103 | |
| 1:400 | Average % | 89 | |
| Range % | 85-93 | |
| 1:800 | Average % | 95 | |
| Range % | 91-99 | |
回收率
| The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-97 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.039 | 2.022 | 2.031 | 1.887 | | | 50 | 1.334 | 1.326 | 1.330 | 1.186 | | | 25 | 0.908 | 0.902 | 0.905 | 0.761 | | | 12.5 | 0.566 | 0.557 | 0.562 | 0.418 | | | 6.25 | 0.358 | 0.364 | 0.361 | 0.217 | | | 3.12 | 0.291 | 0.281 | 0.286 | 0.142 | | | 1.56 | 0.243 | 0.232 | 0.238 | 0.094 | | | 0 | 0.145 | 0.143 | 0.144 | | |
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货期
3-5 working days