产品描述

The Human Protein DJ-1 (PARK7) ELISA kit is the perfect solution for detection of the Parkinson disease protein 7 in human samples. This ELISA kit is ideal for researchers working in the field of cancer and nervous system disease research.

This ELISA kit is designed to detect PARK7 in serum, tissue homogenates, and cell lysates. With a detection range of 0.235 ng/mL-15 ng/mL and a sensitivity of 0.058 ng/mL, this kit provides precise and accurate results in just 1-5 hours.

Using the sandwich assay principle, this ELISA kit provides a quantitative measurement of PARK7 in your samples. With a sample volume of 50-100ul and a detection wavelength of 450 nm, this kit is both versatile and user-friendly.

The Human PARK7 ELISA kit has been validated with citations from over 3 publications, demonstrating its effectiveness in various research settings.

别名

CAP1 ELISA Kit; DJ-1 ELISA Kit; DJ1 ELISA Kit; DJ1 protein ELISA Kit; Epididymis secretory sperm binding protein Li 67p ELISA Kit; FLJ27376 ELISA Kit; FLJ34360 ELISA Kit; FLJ92274 ELISA Kit; HEL S 67p ELISA Kit; Oncogene DJ1 ELISA Kit; OTTHUMP00000001348 ELISA Kit; OTTHUMP00000001349 ELISA Kit; OTTHUMP00000001350 ELISA Kit; OTTHUMP00000001351 ELISA Kit; PARK7 ELISA Kit; PARK7_HUMAN ELISA Kit; Parkinson disease (autosomal recessive, early onset) 7 ELISA Kit; Parkinson disease protein 7 ELISA Kit; Parkinson protein 7 ELISA Kit; Protein DJ-1 ELISA Kit; SP22 ELISA Kit

缩写

PARK7

Uniprot No

Q99497

灵敏度

0.058 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PARK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		80-92
1:2		Average %		97
		Range %		91-105
1:4		Average %		103
		Range %		92-110
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human PARK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 15 1.845 1.752 1.799 1.600 7.5 1.532 1.476 1.504 1.305 3.75 1.112 1.104 1.108 0.909 1.88 0.784 0.739 0.762 0.563 0.94 0.465 0.434 0.450 0.251 0.47 0.376 0.359 0.368 0.169 0.235 0.293 0.290 0.292 0.093 0 0.201 0.197 0.199

货期

3-5 working days