产品描述

This Human KLF4 ELISA Kit was designed for the quantitative measurement of Human KLF4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.6 pg/mL.

别名

Endothelial Kruppel like zinc finger protein ELISA Kit; Epithelial zinc finger protein EZF ELISA Kit; EZF ELISA Kit; GKLF ELISA Kit; gut Kruppel-like factor ELISA Kit; Gut-enriched krueppel-like factor ELISA Kit; KLF ELISA Kit; KLF4 ELISA Kit; KLF4_HUMAN ELISA Kit; Krueppel-like factor 4 ELISA Kit; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) ELISA Kit; Kruppel like factor 4 (gut) ELISA Kit

缩写

KLF4

Uniprot No

O43474

灵敏度

4.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KLF4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		85-92
1:2		Average %		102
		Range %		97-107
1:4		Average %		85
		Range %		90-100
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human KLF4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		83		80-86
EDTA plasma (n=4)		98		95-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1200 2.478 2.498 2.488 2.304   600 2.015 2.136 2.076 1.892   300 1.412 1.465 1.439 1.255   150 0.768 0.758 0.763 0.579   75 0.498 0.472 0.485 0.301   37.5 0.368 0.355 0.362 0.178   18.75 0.271 0.282 0.277 0.093   0 0.183 0.185 0.184

货期

3-5 working days