产品描述
MMP1(基质金属蛋白酶1,Matrix metalloproteinase-1),MMP1是一种由细胞外基质中的成纤维细胞、滑膜细胞、肌肉细胞、成骨细胞等分泌的酶类,属于基质金属蛋白酶家族,能够分解胶原蛋白和基质成分。MMP1在组织重塑、胚胎发育、创伤修复等生理过程中发挥着重要作用。但是,在某些疾病的发生和发展中,如炎症、纤维化、血管新生、肿瘤转移等,MMP1的异常表达可能会导致组织破坏和病情加重。因此,MMP1的测定可以作为某些疾病的辅助诊断指标之一,有一定的临床意义。
华美生物所提供的Human Matrix metalloproteinase 1,MMP-1 ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的MMP1,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
27 kDa interstitial collagenase ELISA Kit; CLG ELISA Kit; CLGN ELISA Kit; collagenase; fibroblast ELISA Kit; collagenase; interstitial ELISA Kit; Fibroblast collagenase ELISA Kit; Interstitial collagenase ELISA Kit; Matrix metallopeptidase 1 (interstitial collagenase) ELISA Kit; Matrix metalloprotease 1 ELISA Kit; Matrix Metalloproteinase 1 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP 1 ELISA Kit; MMP-1 ELISA Kit; MMP1 ELISA Kit; MMP1_HUMAN ELISA Kit; OTTHUMP00000045866 ELISA Kit
缩写
MMP1
Uniprot No
P03956
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
93-105 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
103 |
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| Range % |
95-108 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-102 |
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回收率
| The recovery of human MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.244 |
2.404 |
2.324 |
2.122 |
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| 10 |
1.556 |
1.587 |
1.572 |
1.370 |
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| 5 |
1.062 |
1.081 |
1.072 |
0.870 |
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| 2.5 |
0.709 |
0.736 |
0.723 |
0.521 |
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| 1.25 |
0.476 |
0.499 |
0.488 |
0.286 |
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| 0.625 |
0.374 |
0.397 |
0.386 |
0.184 |
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| 0.312 |
0.330 |
0.336 |
0.333 |
0.131 |
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| 0 |
0.201 |
0.203 |
0.202 |
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货期
3-5 working days