产品描述
This Rat NTF3 ELISA Kit was designed for the quantitative measurement of Rat NTF3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.225 ng/mL.
别名
Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit
缩写
NTF3
Uniprot No
P18280
灵敏度
0.225 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-96 | |
| 1:2 | Average % | 94 | |
| Range % | 89-98 | |
| 1:4 | Average % | 97 | |
| Range % | 93-101 | |
| 1:8 | Average % | 95 | |
| Range % | 89-98 | |
回收率
| The recovery of rat NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 82-94 | |
| EDTA plasma (n=4) | 92 | 86-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 60 | 2.512 | 2.619 | 2.566 | 2.434 | | | 30 | 2.235 | 2.121 | 2.178 | 2.046 | | | 15 | 1.526 | 1.504 | 1.515 | 1.383 | | | 7.5 | 0.812 | 0.823 | 0.818 | 0.686 | | | 3.75 | 0.458 | 0.446 | 0.452 | 0.320 | | | 1.8 | 0.326 | 0.314 | 0.320 | 0.188 | | | 0.9 | 0.221 | 0.227 | 0.224 | 0.092 | | | 0 | 0.133 | 0.131 | 0.132 | | |
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货期
3-5 working days