产品描述
MCP-5(Monocyte chemoattractant protein-5)是一种由单核细胞产生的趋化因子,它可以诱导单核细胞和T细胞的趋化。在炎症和免疫应答过程中,MCP-5的含量通常会升高,其正常范围因人而异,但超过正常范围可能与炎症和免疫疾病相关。
华美生物所提供的Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的MCP-5,其灵敏度为16.82 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
缩写
MCP-5
Uniprot No
Q62401
灵敏度
16.82 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
87 |
|
|
| Range % |
82-91 |
|
|
| 1:2 |
Average % |
94 |
|
|
| Range % |
89-98 |
|
|
| 1:4 |
Average % |
107 |
|
|
| Range % |
103-110 |
|
|
| 1:8 |
Average % |
110 |
|
|
| Range % |
104-114 |
|
|
回收率
| The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
95 |
91-99 |
|
|
| EDTA plasma (n=4) |
103 |
98-108 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 1000 |
2.615 |
2.634 |
2.625 |
2.499 |
|
|
| 500 |
2.177 |
2.012 |
2.095 |
1.969 |
|
|
| 250 |
1.432 |
1.502 |
1.467 |
1.341 |
|
|
| 125 |
0.842 |
0.839 |
0.841 |
0.715 |
|
|
| 62.5 |
0.531 |
0.529 |
0.530 |
0.404 |
|
|
| 31.2 |
0.334 |
0.326 |
0.330 |
0.204 |
|
|
| 15.6 |
0.246 |
0.251 |
0.249 |
0.123 |
|
|
| 0 |
0.125 |
0.127 |
0.126 |
|
|
|
|
货期
3-5 working days