产品描述
This Human INHA ELISA Kit was designed for the quantitative measurement of Human INHA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.4 pg/mL-80 pg/mL and the sensitivity is 0.2 pg/mL.
别名
A inhibin subunit ELISA Kit; A inhibin subunit precursor ELISA Kit; AW55578 ELISA Kit; IHA ELISA Kit; inhA ELISA Kit; INHA_HUMAN ELISA Kit; Inhibin alpha chain ELISA Kit; Inhibin alpha chain precursor ELISA Kit; Inhibin alpha subunit ELISA Kit
缩写
INHA
Uniprot No
P05111
灵敏度
0.2 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human INH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-105 |
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| 1:8 |
Average % |
89 |
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| Range % |
82-95 |
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回收率
| The recovery of human INH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-97 |
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| EDTA plasma (n=4) |
101 |
98-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 80 |
0.184 |
0.186 |
0.185 |
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| 20 |
0.332 |
0.338 |
0.335 |
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| 6.4 |
0.541 |
0.556 |
0.549 |
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| 1.6 |
0.869 |
0.897 |
0.883 |
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| 0.4 |
1.394 |
1.287 |
1.341 |
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| 0 |
2.005 |
2.145 |
2.075 |
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货期
3-5 working days