产品描述
Irisin是一种由肌肉细胞产生的激素,其在运动中释放并能促进脂肪代谢。Irisin的名称来源于“Iris”,即希腊神话中的彩虹女神,因为研究人员发现它具有多种生理功能,包括促进能量消耗、改善糖代谢和增加骨密度等。Irisin的含量与身体代谢状态和肌肉量有关,一些研究表明,Irisin水平在肥胖、糖尿病和骨质疏松等疾病中可能发挥作用。目前,Irisin还处于研究阶段,需要进一步了解其作用机制和生物学功能。
华美生物所提供的Human irisin ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人 血清、血浆、组织匀浆样本中的irisin,其灵敏度为0.78 ng/mL,检测范围为3.12 ng/mL-200 ng/mL。
缩写
irisin
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
90 |
|
|
| Range % |
84-96 |
|
|
| 1:2 |
Average % |
97 |
|
|
| Range % |
91-103 |
|
|
| 1:4 |
Average % |
103 |
|
|
| Range % |
97-109 |
|
|
| 1:8 |
Average % |
89 |
|
|
| Range % |
83-95 |
|
|
回收率
| The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
100 |
94-106 |
|
|
| EDTA plasma (n=4) |
106 |
101-112 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 200 |
2.690 |
2.752 |
2.721 |
2.616 |
|
|
| 100 |
2.402 |
2.371 |
2.387 |
2.282 |
|
|
| 50 |
1.965 |
1.853 |
1.909 |
1.804 |
|
|
| 25 |
1.355 |
1.247 |
1.301 |
1.196 |
|
|
| 12.5 |
0.764 |
0.735 |
0.750 |
0.645 |
|
|
| 6.25 |
0.495 |
0.464 |
0.480 |
0.375 |
|
|
| 3.12 |
0.251 |
0.266 |
0.259 |
0.154 |
|
|
| 0 |
0.106 |
0.103 |
0.105 |
|
|
|
|
货期
3-5 working days