产品描述
This Human MICB ELISA Kit was designed for the quantitative measurement of Human MICB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
MHC class I chain related protein B ELISA Kit; MHC class I chain related protein B2 ELISA Kit; MHC class I like molecule PERB11.2 IMX ELISA Kit; MHC class I mic B antigen ELISA Kit; MHC class I polypeptide related sequence B ELISA Kit; MHC class I polypeptide-related sequence B ELISA Kit; MIC B ELISA Kit; MIC-B ELISA Kit; MICB ELISA Kit; MICB_HUMAN ELISA Kit; PERB11.2 ELISA Kit; Stress inducible class I homolog ELISA Kit
缩写
MICB
Uniprot No
Q29980
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MICB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
99 |
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| Range % |
93-104 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-98 |
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回收率
| The recovery of human MICB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
94-101 |
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| EDTA plasma (n=4) |
86 |
82-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.364 |
2.477 |
2.421 |
2.267 |
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| 1000 |
1.767 |
1.654 |
1.711 |
1.557 |
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| 500 |
1.081 |
1.136 |
1.109 |
0.955 |
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| 250 |
0.713 |
0.724 |
0.719 |
0.565 |
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| 125 |
0.442 |
0.455 |
0.449 |
0.295 |
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| 62.5 |
0.371 |
0.367 |
0.369 |
0.215 |
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| 31.25 |
0.252 |
0.259 |
0.256 |
0.102 |
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| 0 |
0.153 |
0.154 |
0.154 |
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货期
3-5 working days