产品描述
This Sheep IL1B ELISA Kit was designed for the quantitative measurement of Sheep IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写
IL1B
Uniprot No
P21621
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 83-90 | |
| 1:2 | Average % | 98 | |
| Range % | 92-102 | |
| 1:4 | Average % | 100 | |
| Range % | 96-104 | |
| 1:8 | Average % | 94 | |
| Range % | 90-96 | |
回收率
| The recovery of sheep IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-97 | |
| EDTA plasma (n=4) | 97 | 94-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.487 | 2.456 | 2.472 | 2.371 | | | 500 | 2.031 | 2.000 | 2.016 | 1.915 | | | 250 | 1.614 | 1.568 | 1.591 | 1.490 | | | 125 | 1.105 | 1.023 | 1.064 | 0.963 | | | 62.5 | 0.703 | 0.689 | 0.696 | 0.595 | | | 31.2 | 0.421 | 0.402 | 0.412 | 0.311 | | | 15.6 | 0.275 | 0.262 | 0.269 | 0.168 | | | 0 | 0.103 | 0.099 | 0.101 | | |
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货期
3-5 working days