产品描述

This Human KLK5 ELISA Kit was designed for the quantitative measurement of Human KLK5 protein in serum, plasma, cell culture supernates, tissue homogenates, saliva. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

HK5 ELISA Kit; Kallikrein 5 ELISA Kit; Kallikrein L2 ELISA Kit; Kallikrein like 2 ELISA Kit; Kallikrein like protein 2 ELISA Kit; Kallikrein related peptidase 5 ELISA Kit; Kallikrein-5 ELISA Kit; Kallikrein-like protein 2 ELISA Kit; Kallikrein5 ELISA Kit; KLK 5 ELISA Kit; KLK L2 ELISA Kit; KLK-L2 ELISA Kit; KLK5 ELISA Kit; KLK5_HUMAN ELISA Kit; KLKL 2 ELISA Kit; KLKL2 ELISA Kit; SCTE ELISA Kit; Stratum corneum tryptic enzyme ELISA Kit; UNQ570/PRO1132 ELISA Kit

缩写

KLK5

Uniprot No

Q9Y337

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		89-98
1:2		Average %		98
		Range %		93-104
1:4		Average %		95
		Range %		89-99
1:8		Average %		94
		Range %		87-98

回收率

The recovery of human KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-98
EDTA plasma (n=4)		93		87-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 2.624 2.524 2.574 2.461 2000 2.096 1.987 2.042 1.929 1000 1.381 1.364 1.373 1.260 500 0.719 0.742 0.731 0.618 250 0.409 0.434 0.422 0.309 125 0.297 0.282 0.290 0.177 62.5 0.210 0.215 0.213 0.100 0 0.112 0.113 0.113

货期

3-5 working days