产品描述

The human IL-33 ELISA Kit is engineered for accurate measurement of human IL-33 levels from samples including serum, plasma, or urine. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-33 content in the sample. The color intensity is positively correlated with IL-33 content in the sample. The IL-33 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

IL-33 is constitutively expressed in the nucleus of epithelial, endothelial cells, and fibroblasts and participates in innate and adaptive immunity by enhancing Th2 cytokines generation. It is secreted secondary to cell apoptosis and necrosis. It functions as an 'alarmin' released following cell necrosis to alert the immune system to tissue damage or stress. IL-33 binds to a heterodimeric receptor ST2/IL-1R4 and forms a complex with the IL-1RAcP, recruiting the adaptor protein MyD88 and activating transcription factors such as NF-κB via TRAF6, IRAK-1/4, and MAP kinases and inducing the generation of inflammatory mediators. IL-33 drives the pathogenesis of Th2-related diseases such as asthma, atopic dermatitis, and anaphylaxis because it strongly induces Th2 cytokine production. However, IL-33 has shown various protective effects on cardiovascular diseases such as atherosclerosis, obesity, and type 2 diabetes.

别名

C9orf26 ELISA Kit; CHROMOSOME 9 OPEN READING FRAME 26 ELISA Kit; DKFZp586H0523 ELISA Kit; DVS27 ELISA Kit; DVS27 related protein ELISA Kit; IL 1F11 ELISA Kit; IL 33 ELISA Kit; IL-1F11 ELISA Kit; IL-33 ELISA Kit; IL1F11 ELISA Kit; IL33 ELISA Kit; IL33_HUMAN ELISA Kit; Interleukin 1 family member 11 ELISA Kit; Interleukin 33 ELISA Kit; INTERLEUKIN 33 NFHEV ELISA Kit; Interleukin 33 precursor ELISA Kit; Interleukin-1 family member 11 ELISA Kit; Interleukin-33 (109-270) ELISA Kit; Interleukin33 ELISA Kit; NF HEV ELISA Kit; NF-HEV ELISA Kit; NFEHEV ELISA Kit; NFHEV ELISA Kit; Nuclear factor for high endothelial venules ELISA Kit; Nuclear factor from high endothelial venules ELISA Kit; OTTHUMP00000021041 ELISA Kit; RP11 575C20.2 ELISA Kit

缩写

IL33

Uniprot No

O95760

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		80-94
1:2		Average %		96
		Range %		90-99
1:4		Average %		106
		Range %		92-115
1:8		Average %		95
		Range %		86-100

回收率

The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-99
EDTA plasma (n=4)		97		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 1.894 1.752 1.823 1.728 500 1.275 1.186 1.231 1.136 250 0.734 0.698 0.716 0.621 125 0.421 0.409 0.415 0.320 62.5 0.245 0.239 0.242 0.147 31.2 0.165 0.159 0.162 0.067 15.6 0.112 0.109 0.111 0.016 0 0.095 0.094 0.095

货期

3-5 working days