产品描述
This Rat IL-9 ELISA Kit was designed for the quantitative measurement of Rat IL-9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
缩写
IL-9
Uniprot No
D4A8I9
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-103 | |
| 1:2 | Average % | 92 | |
| Range % | 88-96 | |
| 1:4 | Average % | 95 | |
| Range % | 90-99 | |
| 1:8 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of rat IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-95 | |
| EDTA plasma (n=4) | 98 | 93-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.489 | 2.353 | 2.421 | 2.322 | | | 500 | 1.954 | 2.076 | 2.015 | 1.916 | | | 250 | 1.481 | 1.444 | 1.463 | 1.364 | | | 125 | 0.823 | 0.806 | 0.815 | 0.716 | | | 62.5 | 0.402 | 0.417 | 0.410 | 0.311 | | | 31.2 | 0.215 | 0.229 | 0.222 | 0.123 | | | 15.6 | 0.160 | 0.154 | 0.157 | 0.058 | | | 0 | 0.096 | 0.102 | 0.099 | | |
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货期
3-5 working days