产品描述
This Human ITGA2B ELISA Kit was designed for the quantitative measurement of Human ITGA2B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
antigen CD41 ELISA Kit; BDPLT16 ELISA Kit; BDPLT2 ELISA Kit; CD41 ELISA Kit; CD41B ELISA Kit; form 2 ELISA Kit; GP2B ELISA Kit; GPalpha IIb ELISA Kit; GPIIb ELISA Kit; GT ELISA Kit; GTA ELISA Kit; HPA3 ELISA Kit; Integrin alpha 2b ELISA Kit; Integrin alpha IIb ELISA Kit; Integrin alpha-IIb light chain ELISA Kit; Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) ELISA Kit; ITA2B_HUMAN ELISA Kit; Itga2b ELISA Kit; ITGAB ELISA Kit; platelet fibrinogen receptor, alpha subunit ELISA Kit; platelet glycoprotein IIb of IIb/IIIa complex ELISA Kit; Platelet membrane glycoprotein IIb ELISA Kit; platelet specific antigen BAK ELISA Kit; PPP1R93 ELISA Kit
缩写
ITGA2B
Uniprot No
P08514
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 103 | | |
| Range % | 93-107 | | |
| 1:2 | Average % | 98 | | |
| Range % | 90-103 | | |
| 1:4 | Average % | 89 | | |
| Range % | 81-92 | | |
| 1:8 | Average % | 96 | | |
| Range % | 88-100 | | |
回收率
| The recovery of human ITGA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 83-94 | | |
| EDTA plasma (n=4) | 104 | 94-109 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 2.293 | 2.144 | 2.219 | 2.062 | | | | 5 | 1.325 | 1.274 | 1.300 | 1.143 | | | | 2.5 | 0.881 | 0.867 | 0.874 | 0.717 | | | | 1.25 | 0.513 | 0.523 | 0.518 | 0.361 | | | | 0.625 | 0.346 | 0.351 | 0.349 | 0.192 | | | | 0.312 | 0.289 | 0.280 | 0.285 | 0.128 | | | | 0.156 | 0.212 | 0.219 | 0.216 | 0.059 | | | | 0 | 0.159 | 0.155 | 0.157 | | | |
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货期
3-5 working days