产品描述
This Pig IL2 ELISA Kit was designed for the quantitative measurement of Pig IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写
IL2
Uniprot No
P26891
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 80-99 | |
| 1:2 | Average % | 86 | |
| Range % | 82-91 | |
| 1:4 | Average % | 91 | |
| Range % | 86-97 | |
| 1:8 | Average % | 94 | |
| Range % | 88-98 | |
回收率
| The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-102 | |
| EDTA plasma (n=4) | 96 | 91-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.884 | 2.915 | 2.900 | 2.764 | | | 500 | 2.123 | 2.104 | 2.114 | 1.978 | | | 250 | 1.249 | 1.272 | 1.261 | 1.125 | | | 125 | 0.818 | 0.846 | 0.832 | 0.696 | | | 62.5 | 0.492 | 0.440 | 0.466 | 0.330 | | | 31.2 | 0.314 | 0.322 | 0.318 | 0.182 | | | 15.6 | 0.263 | 0.258 | 0.261 | 0.125 | | | 0 | 0.135 | 0.136 | 0.136 | | |
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货期
3-5 working days