产品描述

This Human IL9 ELISA Kit was designed for the quantitative measurement of Human IL9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

Cytokine P40 ELISA Kit; Homolog of mouse T cell and mast cell growth factor 40 ELISA Kit; HP40 ELISA Kit; IL 9 ELISA Kit; IL-9 ELISA Kit; Il9 ELISA Kit; IL9_HUMAN ELISA Kit; Interleukin 9 ELISA Kit; Interleukin-9 ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Megakaryoblast growth factor ELISA Kit; P40 ELISA Kit; p40 cytokine ELISA Kit; p40 T cell and mast cell growth factor ELISA Kit; T cell growth factor 3 ELISA Kit; T cell growth factor p40 ELISA Kit; T-cell growth factor P40 ELISA Kit; TCGF 3 ELISA Kit

缩写

IL9

Uniprot No

P15248

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		100
		Range %		97-103
1:2		Average %		99
		Range %		94-103
1:4		Average %		101
		Range %		98-106
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		83		80-86
EDTA plasma (n=4)		94		89-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.403 2.498 2.451 2.306 500 1.520 1.568 1.544 1.399 250 0.845 0.898 0.872 0.727 125 0.523 0.568 0.546 0.401 62.5 0.365 0.367 0.366 0.221 31.2 0.282 0.271 0.277 0.132 15.6 0.221 0.219 0.220 0.075 0 0.143 0.146 0.145

货期

3-5 working days