产品描述
IL6(Interleukin-6),是一种细胞因子,能够影响多种免疫反应、代谢调节以及细胞增殖等生物学过程。IL6在炎症反应中发挥着重要作用,能够吸引中性粒细胞、单核细胞等炎性细胞浸润到炎症部位,同时还能刺激肝脏合成急性反应蛋白等。人体内IL6的含量与炎症反应、肿瘤等疾病的发生密切相关。
华美生物所提供的Rabbit Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测兔血清、血浆、组织匀浆样本中的IL6,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
Q9MZR1
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
99 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
101 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.840 |
2.785 |
2.813 |
2.710 |
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| 500 |
2.316 |
2.223 |
2.270 |
2.167 |
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| 250 |
1.580 |
1.567 |
1.574 |
1.471 |
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| 125 |
0.935 |
0.874 |
0.905 |
0.802 |
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| 62.5 |
0.479 |
0.462 |
0.471 |
0.368 |
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| 31.2 |
0.266 |
0.254 |
0.260 |
0.157 |
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| 15.6 |
0.170 |
0.158 |
0.164 |
0.061 |
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| 0 |
0.107 |
0.099 |
0.103 |
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货期
3-5 working days