产品描述

This Human MYCN ELISA Kit was designed for the quantitative measurement of Human MYCN protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

bHLHe37 ELISA Kit; Class E basic helix-loop-helix protein 37 ELISA Kit; MODED ELISA Kit; mycn ELISA Kit; MYCN_HUMAN ELISA Kit; N myc ELISA Kit; N myc proto oncogene protein ELISA Kit; N-myc proto-oncogene protein ELISA Kit; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene ELISA Kit; Neuroblastoma MYC oncogene ELISA Kit; NMYC ELISA Kit; NMYC proto oncogene protein ELISA Kit; ODED ELISA Kit; Oncogene NMYC ELISA Kit; pp65/67 ELISA Kit; V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog ELISA Kit; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived ELISA Kit; v myc myelocytomatosis viral related oncogene neuroblastoma derived ELISA Kit

缩写

MYCN

Uniprot No

P04198

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MYCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		104
		Range %		99-108
1:2		Average %		97
		Range %		92-102
1:4		Average %		95
		Range %		90-99
1:8		Average %		94
		Range %		86-98

回收率

The recovery of human MYCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		99		94-101
EDTA plasma (n=4)		86		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.414 2.328 2.371 2.213 500 1.397 1.492 1.445 1.287 250 0.897 0.857 0.877 0.719 125 0.577 0.568 0.573 0.415 62.5 0.375 0.364 0.370 0.212 31.2 0.281 0.296 0.289 0.131 15.6 0.228 0.215 0.222 0.064 0 0.159 0.157 0.158

货期

3-5 working days