产品描述
This Human LSR ELISA Kit was designed for the quantitative measurement of Human LSR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
ILDR3 ELISA Kit; Immunoglobulin like domain containing receptor 3 ELISA Kit; Lipolysis Stimulated Lipoprotein Receptor ELISA Kit; Lipolysis stimulated remnant ELISA Kit; Lipolysis-stimulated lipoprotein receptor ELISA Kit; LISCH ELISA Kit; LISCH protein ELISA Kit; LISCH7 ELISA Kit; Liver specific bHLH Zip transcription factor ELISA Kit; LSR ELISA Kit; LSR_HUMAN ELISA Kit; MGC10659 ELISA Kit; MGC48312 ELISA Kit; MGC48503 ELISA Kit
缩写
LSR
Uniprot No
Q86X29
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human LSR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 99 | |
| Range % | 90-103 | |
| 1:2 | Average % | 96 | |
| Range % | 90-99 | |
| 1:4 | Average % | 91 | |
| Range % | 85-95 | |
| 1:8 | Average % | 92 | |
| Range % | 84-97 | |
回收率
| The recovery of human LSR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 83-97 | |
| EDTA plasma (n=4) | 101 | 91-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.804 | 2.722 | 2.763 | 2.593 | | | 200 | 2.201 | 2.129 | 2.165 | 1.995 | | | 100 | 1.470 | 1.442 | 1.456 | 1.286 | | | 50 | 0.770 | 0.756 | 0.763 | 0.593 | | | 25 | 0.500 | 0.512 | 0.506 | 0.336 | | | 12.5 | 0.304 | 0.300 | 0.302 | 0.132 | | | 6.25 | 0.227 | 0.235 | 0.231 | 0.061 | | | 0 | 0.168 | 0.172 | 0.170 | | |
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货期
3-5 working days