产品描述
IL18(Interleukin-18,白细胞介素18)是一种由多种免疫细胞产生的细胞因子,属于IL-1家族成员。IL-18主要由巨噬细胞、树突细胞和上皮细胞等细胞合成,并参与了免疫细胞之间的相互作用和免疫炎症的调节。IL-18在免疫细胞的激活、增殖和分化等生物学过程中发挥着重要作用,对免疫应答和炎症反应起着调节作用。此外,IL-18还与多种疾病的发生和发展相关,如炎症性肠病、自身免疫病和肿瘤等。
华美生物所提供的Rat Interleukin 18,IL-18 Elisa Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清样本中的IL18,其灵敏度为0.039 ng/mL,检测范围为0.156 ng/mL-10 ng/mL。
别名
Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写
IL18
Uniprot No
P97636
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
89 |
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| Range % |
84-95 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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回收率
| The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
102 |
98-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.357 |
2.387 |
2.372 |
2.218 |
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| 5 |
1.686 |
1.699 |
1.693 |
1.539 |
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| 2.5 |
1.064 |
1.015 |
1.040 |
0.886 |
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| 1.25 |
0.687 |
0.654 |
0.671 |
0.517 |
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| 0.625 |
0.382 |
0.398 |
0.390 |
0.236 |
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| 0.312 |
0.289 |
0.270 |
0.280 |
0.126 |
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| 0.156 |
0.221 |
0.235 |
0.228 |
0.074 |
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| 0 |
0.151 |
0.157 |
0.154 |
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货期
3-5 working days