产品描述
This Human protein Red (IK) ELISA Kit was designed for the quantitative measurement of Human protein Red (IK) protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
别名
chondrosarcoma associated protein 2 ELISA Kit; CSA2 ELISA Kit; Cytokine IK ELISA Kit; IK ELISA Kit; IK cytokine down regulator of HLA II ELISA Kit; IK factor ELISA Kit; IK Gene ELISA Kit; prer protein ELISA Kit; Protein Red ELISA Kit; Protein RER ELISA Kit; RD element ELISA Kit; RED protein ELISA Kit; RED_HUMAN ELISA Kit; RER ELISA Kit
缩写
IK
Uniprot No
Q13123
灵敏度
6.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 82-94 | | |
| 1:2 | Average % | 101 | | |
| Range % | 98-104 | | |
| 1:4 | Average % | 92 | | |
| Range % | 85-96 | | |
| 1:8 | Average % | 98 | | |
| Range % | 94-102 | | |
回收率
| The recovery of human IK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 85-94 | | |
| EDTA plasma (n=4) | 93 | 86-97 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1600 | 2.909 | 2.965 | 2.937 | 2.763 | | | | 800 | 2.215 | 2.332 | 2.274 | 2.100 | | | | 400 | 1.462 | 1.487 | 1.475 | 1.301 | | | | 200 | 0.900 | 0.923 | 0.912 | 0.738 | | | | 100 | 0.555 | 0.564 | 0.560 | 0.386 | | | | 50 | 0.363 | 0.378 | 0.371 | 0.197 | | | | 25 | 0.243 | 0.249 | 0.246 | 0.072 | | | | 0 | 0.173 | 0.174 | 0.174 | | | |
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货期
3-5 working days