产品描述
Kim-1,肾上皮细胞跨膜蛋白1(Kidney Injury Molecule-1),属于肾小管上皮细胞表面分子,是急性肾损伤的重要标志物。Kim-1在正常情况下表达较低,但在肾小管上皮细胞受到损伤后,其表达会显著上调。因此,Kim-1可作为早期诊断急性肾损伤的敏感指标,对于肾脏疾病的诊断和监测具有重要意义。
华美生物所提供的Human Kidney injury molecule 1,Kim-1 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、尿液、组织匀浆样本中的Kim-1,其灵敏度为0.043 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
CD365 ELISA Kit; HAVCR 1 ELISA Kit; HAVCR ELISA Kit; HAVcr-1 ELISA Kit; Havcr1 ELISA Kit; Hepatitis A virus cellular receptor 1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobin domain and mucin domain protein 1 ELISA Kit; T cell immunoglobulin mucin family member 1 ELISA Kit; T cell immunoglobulin mucin receptor 1 ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; T-cell membrane protein 1 ELISA Kit; TIM ELISA Kit; TIM-1 ELISA Kit; TIM1 ELISA Kit; TIMD 1 ELISA Kit; TIMD-1 ELISA Kit; TIMD1 ELISA Kit; TIMD1_HUMAN ELISA Kit
缩写
Kim-1
Uniprot No
Q96D42
灵敏度
0.043 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-96 |
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| 1:2 |
Average % |
104 |
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| Range % |
99-109 |
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| 1:4 |
Average % |
100 |
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| Range % |
94-106 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-98 |
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回收率
| The recovery of human Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
96-108 |
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| EDTA plasma (n=4) |
90 |
84-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.409 |
2.565 |
2.487 |
2.317 |
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| 10 |
1.599 |
1.518 |
1.559 |
1.389 |
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| 5 |
1.012 |
1.003 |
1.008 |
0.838 |
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| 2.5 |
0.587 |
0.578 |
0.583 |
0.413 |
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| 1.25 |
0.433 |
0.463 |
0.448 |
0.278 |
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| 0.625 |
0.302 |
0.304 |
0.303 |
0.133 |
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| 0.312 |
0.231 |
0.235 |
0.233 |
0.063 |
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| 0 |
0.174 |
0.165 |
0.170 |
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货期
3-5 working days