产品描述

This Human MASP1 ELISA Kit was designed for the quantitative measurement of Human MASP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

别名

MASP1 ELISA Kit; CRARF ELISA Kit; CRARF1 ELISA Kit; PRSS5Mannan-binding lectin serine protease 1 ELISA Kit; EC 3.4.21.- ELISA Kit; Complement factor MASP-3 ELISA Kit; Complement-activating component of Ra-reactive factor ELISA Kit; Mannose-binding lectin-associated serine protease 1 ELISA Kit; MASP-1 ELISA Kit; Mannose-binding protein-associated serine protease ELISA Kit; Ra-reactive factor serine protease p100 ELISA Kit; RaRF ELISA Kit; Serine protease 5) [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain] ELISA Kit

缩写

MASP1

Uniprot No

P48740

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		96
		Range %		91-99
1:200		Average %		100
		Range %		95-104
1:400		Average %		94
		Range %		89-98
1:800		Average %		99
		Range %		93-103

回收率

The recovery of human MASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		88-97
EDTA plasma (n=4)		94		90-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 200 2.797 2.646 2.722 2.616 100 2.026 2.000 2.013 1.907 50 1.255 1.244 1.250 1.144 25 0.701 0.698 0.700 0.594 12.5 0.416 0.403 0.410 0.304 6.25 0.302 0.297 0.300 0.194 3.12 0.187 0.196 0.192 0.086 0 0.108 0.104 0.106

货期

3-5 working days