产品描述

This Human LILRB4 ELISA Kit was designed for the quantitative measurement of Human LILRB4 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

CD85 antigen-like family member K ELISA Kit; CD85k ELISA Kit; HM18 ELISA Kit; ILT-3 ELISA Kit; ILT3 ELISA Kit; Immunoglobulin-like transcript 3 ELISA Kit; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 ELISA Kit; Leukocyte immunoglobulin-like receptor 5 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 4 ELISA Kit; LILRB4 ELISA Kit; LILRB5 ELISA Kit; LIR-5 ELISA Kit; LIR5 ELISA Kit; LIRB4_HUMAN ELISA Kit; Monocyte inhibitory receptor HM18 ELISA Kit

缩写

LILRB4

Uniprot No

Q8NHJ6

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LILRB4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-98
1:2		Average %		97
		Range %		93-102
1:4		Average %		84
		Range %		81-89
1:8		Average %		88
		Range %		83-95

回收率

The recovery of human LILRB4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		98-104
EDTA plasma (n=4)		92		89-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.188 2.288 2.238 2.113 1000 1.833 1.899 1.866 1.741 500 1.314 1.392 1.353 1.228 250 0.954 0.945 0.950 0.825 125 0.478 0.460 0.469 0.344 62.5 0.320 0.344 0.332 0.207 31.25 0.211 0.212 0.212 0.087 0 0.120 0.129 0.125

货期

3-5 working days