产品描述
IGFBP2 (Insulin-like growth factor-binding protein 2)是一种蛋白质,主要作用是与IGF(胰岛素样生长因子)结合,调控细胞生长和代谢。IGFBP2的高表达与许多癌症相关,例如卵巢癌、结直肠癌和肺癌等。此外,IGFBP2在神经发育和神经退行性疾病中也起着重要作用。生物学含义上,IGFBP2可作为肿瘤标志物用于临床诊断和治疗。在正常体内,IGFBP2含量较低,但在癌症患者中常常高表达。
华美生物所提供的Human insulin-like growth factors binding protein 2,IGFBP-2 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的IGFBP2,其灵敏度为0.195 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
BP 2 ELISA Kit; BP2 ELISA Kit; IBP 2 ELISA Kit; IBP-2 ELISA Kit; IBP2 ELISA Kit; IBP2_HUMAN ELISA Kit; IGF binding protein 2 ELISA Kit; IGF BP53 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP 2 ELISA Kit; IGFBP-2 ELISA Kit; IGFBP2 ELISA Kit; IGFBP53 ELISA Kit; Insulin like growth factor binding protein 2 36kDa ELISA Kit; Insulin like growth factor binding protein 2 ELISA Kit; Insulin like growth factor-binding protein 2 precursor ELISA Kit; Insulin-like growth factor-binding protein 2 ELISA Kit
缩写
IGFBP2
Uniprot No
P18065
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
97 |
|
| Range % |
91-105 |
|
| 1:10 |
Average % |
95 |
|
| Range % |
92-98 |
|
| 1:20 |
Average % |
92 |
|
| Range % |
86-99 |
|
| 1:40 |
Average % |
94 |
|
| Range % |
89-100 |
|
回收率
| The recovery of human IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
89-101 |
|
| EDTA plasma (n=4) |
94 |
88-99 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.935 |
2.952 |
2.944 |
2.767 |
|
| 25 |
2.026 |
2.076 |
2.051 |
1.874 |
|
| 12.5 |
1.088 |
1.046 |
1.067 |
0.890 |
|
| 6.25 |
0.592 |
0.583 |
0.588 |
0.411 |
|
| 3.12 |
0.361 |
0.337 |
0.349 |
0.172 |
|
| 1.56 |
0.278 |
0.259 |
0.269 |
0.092 |
|
| 0.78 |
0.232 |
0.229 |
0.231 |
0.054 |
|
| 0 |
0.176 |
0.177 |
0.177 |
|
|
|
货期
3-5 working days