产品描述
This Pig IL1RN ELISA Kit was designed for the quantitative measurement of Pig IL1RN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
别名
IL1RN ELISA kit; IRAP1Interleukin-1 receptor antagonist protein ELISA kit; IL-1RN ELISA kit; IL-1ra ELISA kit; IRAP ELISA kit; IL1 inhibitor ELISA kit
缩写
IL1RN
Uniprot No
Q29056
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 106 | | |
| Range % | 93-110 | | |
| 1:2 | Average % | 101 | | |
| Range % | 91-105 | | |
| 1:4 | Average % | 97 | | |
| Range % | 87-101 | | |
| 1:8 | Average % | 91 | | |
| Range % | 84-95 | | |
回收率
| The recovery of pig IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 85-96 | | |
| EDTA plasma (n=4) | 108 | 98-112 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 4000 | 2.243 | 2.187 | 2.215 | 2.032 | | | | 2000 | 1.441 | 1.366 | 1.404 | 1.221 | | | | 1000 | 0.986 | 0.954 | 0.970 | 0.787 | | | | 500 | 0.658 | 0.647 | 0.653 | 0.470 | | | | 250 | 0.422 | 0.402 | 0.412 | 0.229 | | | | 125 | 0.352 | 0.343 | 0.348 | 0.165 | | | | 62.5 | 0.277 | 0.263 | 0.270 | 0.087 | | | | 0 | 0.186 | 0.180 | 0.183 | | | |
|
货期
3-5 working days