产品描述
This Bovine IL10 ELISA Kit was designed for the quantitative measurement of Bovine IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.
别名
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写
IL10
Uniprot No
P43480
灵敏度
2.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
96 |
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| Range % |
87-102 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-104 |
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回收率
| The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
95-100 |
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| EDTA plasma (n=4) |
96 |
92-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.098 |
0.095 |
0.097 |
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| 250 |
0.301 |
0.285 |
0.293 |
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| 80 |
0.487 |
0.458 |
0.473 |
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| 20 |
0.972 |
0.975 |
0.974 |
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| 5 |
1.364 |
1.316 |
1.340 |
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| 0 |
2.128 |
2.211 |
2.170 |
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货期
3-5 working days