产品描述
This Mouse Itgam/CD11b ELISA Kit was designed for the quantitative measurement of Mouse Itgam/CD11b protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
缩写
Itgam/CD11b
Uniprot No
P05555
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 86 | | |
| Range % | 81-90 | | |
| 1:200 | Average % | 103 | | |
| Range % | 99-108 | | |
| 1:400 | Average % | 97 | | |
| Range % | 92-101 | | |
| 1:800 | Average % | 90 | | |
| Range % | 86-94 | | |
回收率
| The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 90-100 | | |
| EDTA plasma (n=4) | 88 | 84-92 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.603 | 2.527 | 2.565 | 2.376 | | | | 500 | 1.920 | 1.865 | 1.893 | 1.704 | | | | 250 | 1.367 | 1.441 | 1.404 | 1.215 | | | | 125 | 0.905 | 0.928 | 0.917 | 0.728 | | | | 62.5 | 0.586 | 0.574 | 0.580 | 0.391 | | | | 31.2 | 0.439 | 0.419 | 0.429 | 0.240 | | | | 15.6 | 0.291 | 0.304 | 0.298 | 0.109 | | | | 0 | 0.186 | 0.192 | 0.189 | | | |
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货期
3-5 working days