产品描述
This Human ITGB2 ELISA Kit was designed for the quantitative measurement of Human ITGB2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
ITGB2; CD18; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD antigen CD18
缩写
ITGB2
Uniprot No
P05107
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITG β2/CD11+CD18CD18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
91 |
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| Range % |
84-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-98 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-102 |
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回收率
| The recovery of human ITG β2/CD11+CD18CD18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
96 |
87-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
1.631 |
1.609 |
1.620 |
1.523 |
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| 500 |
1.365 |
1.318 |
1.342 |
1.245 |
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| 250 |
0.952 |
0.943 |
0.948 |
0.851 |
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| 125 |
0.650 |
0.635 |
0.643 |
0.546 |
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| 62.5 |
0.381 |
0.377 |
0.379 |
0.282 |
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| 31.2 |
0.267 |
0.259 |
0.263 |
0.166 |
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| 15.6 |
0.185 |
0.191 |
0.188 |
0.091 |
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| 0 |
0.095 |
0.099 |
0.097 |
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货期
3-5 working days