产品描述
IL-2 (Interleukin-2),是一种介导T细胞增殖和分化的白细胞介素,对于机体免疫功能的调节起着重要作用。IL-2具有促进T细胞增殖、增强T细胞活性、诱导自身再生及增殖等生物学功能,对于免疫细胞的分化和增殖调节至关重要。在某些肿瘤和自身免疫性疾病的治疗中,IL-2也有着广泛的应用。测定IL-2水平可作为评估患者的免疫功能状态的一种指标。
华美生物所提供的Chicken Interleukin 2,IL-2 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鸡血清、血浆、组织匀浆样本中的IL-2,其灵敏度为0.04 pg/mL,检测范围为0.18 pg/mL-12 pg/mL。
缩写
IL-2
Uniprot No
O73883
灵敏度
0.04 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
85-96 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-97 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-106 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-104 |
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回收率
| The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
92-109 |
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| EDTA plasma (n=4) |
96 |
90-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 12 |
2.151 |
2.198 |
2.175 |
2.072 |
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| 6 |
1.835 |
1.869 |
1.852 |
1.749 |
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| 3 |
1.457 |
1.487 |
1.472 |
1.369 |
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| 1.5 |
1.098 |
1.051 |
1.075 |
0.972 |
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| 0.75 |
0.698 |
0.682 |
0.690 |
0.587 |
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| 0.38 |
0.449 |
0.460 |
0.455 |
0.352 |
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| 0.18 |
0.236 |
0.222 |
0.229 |
0.126 |
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| 0 |
0.102 |
0.104 |
0.103 |
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货期
3-5 working days