产品描述
This Mouse MT-2 ELISA Kit was designed for the quantitative measurement of Mouse MT-2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Mt2 ELISA Kit; Metallothionein-2 ELISA Kit; MT-2 ELISA Kit; Metallothionein-II ELISA Kit; MT-II ELISA Kit
缩写
MT-2
Uniprot No
P02798
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 91-102 | |
| 1:2 | Average % | 102 | |
| Range % | 99-105 | |
| 1:4 | Average % | 89 | |
| Range % | 86-93 | |
| 1:8 | Average % | 94 | |
| Range % | 92-97 | |
回收率
| The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 103 | 101-106 | |
| EDTA plasma (n=4) | 95 | 92-109 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 1.975 | 2.022 | 1.999 | 1.842 | | | 500 | 1.362 | 1.377 | 1.370 | 1.213 | | | 250 | 0.811 | 0.862 | 0.837 | 0.680 | | | 125 | 0.433 | 0.439 | 0.436 | 0.279 | | | 62.5 | 0.304 | 0.330 | 0.317 | 0.160 | | | 31.2 | 0.262 | 0.274 | 0.268 | 0.111 | | | 15.6 | 0.214 | 0.220 | 0.217 | 0.060 | | | 0 | 0.163 | 0.150 | 0.157 | | |
|
货期
3-5 working days