产品描述

This Human NOD1 ELISA Kit was designed for the quantitative measurement of Human NOD1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

CARD 4 ELISA Kit; CARD4 ELISA Kit; Caspase recruitment domain 4 ELISA Kit; Caspase recruitment domain containing protein 4 ELISA Kit; Caspase recruitment domain family member 4 ELISA Kit; Caspase recruitment domain protein 4 ELISA Kit; Caspase recruitment domain-containing protein 4 ELISA Kit; CLR 7.1 ELISA Kit; CLR7.1 ELISA Kit; NLR family CARD domain containing 1 ELISA Kit; NLRC 1 ELISA Kit; NLRC1 ELISA Kit; NOD 1 ELISA Kit; Nod1 ELISA Kit; NOD1 protein ELISA Kit; NOD1_HUMAN ELISA Kit; Nucleotide binding oligomerization domain containing 1 ELISA Kit; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 ELISA Kit; Nucleotide-binding oligomerization domain-containing protein 1 ELISA Kit; Protein Nod1 ELISA Kit

缩写

NOD1

Uniprot No

Q9Y239

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		88-100
1:2		Average %		97
		Range %		92-105
1:4		Average %		95
		Range %		82-104
1:5		Average %		96
		Range %		90-102

回收率

The recovery of human NOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		95-105
EDTA plasma (n=4)		98		95-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.213 2.075 2.144 1.996 500 1.436 1.396 1.416 1.268 250 0.824 0.816 0.820 0.672 125 0.468 0.431 0.450 0.302 62.5 0.369 0.351 0.360 0.212 31.2 0.271 0.262 0.267 0.119 15.6 0.203 0.198 0.201 0.053 0 0.149 0.147 0.148

货期

3-5 working days