产品描述
This Mouse NT-proBNP ELISA Kit was designed for the quantitative measurement of Mouse NT-proBNP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
缩写
NT-proBNP
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 90 | | |
| Range % | 85-96 | | |
| 1:2 | Average % | 96 | | |
| Range % | 91-105 | | |
| 1:4 | Average % | 89 | | |
| Range % | 85-99 | | |
| 1:8 | Average % | 93 | | |
| Range % | 83-98 | | |
回收率
| The recovery of mouse NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 87-105 | | |
| EDTA plasma (n=4) | 95 | 91-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 2.235 | 2.199 | 2.217 | 2.090 | | | | 5 | 1.884 | 1.749 | 1.817 | 1.690 | | | | 2.5 | 1.462 | 1.375 | 1.419 | 1.292 | | | | 1.25 | 0.978 | 1.035 | 1.007 | 0.880 | | | | 0.625 | 0.552 | 0.549 | 0.551 | 0.424 | | | | 0.312 | 0.289 | 0.284 | 0.287 | 0.160 | | | | 0.156 | 0.203 | 0.211 | 0.207 | 0.080 | | | | 0 | 0.128 | 0.125 | 0.127 | | | |
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货期
3-5 working days