产品描述
This Human LL-37 ELISA Kit was designed for the quantitative measurement of Human LL-37 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
缩写
LL-37
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human LL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 85 | |
| Range % | 80-92 | |
| 1:10 | Average % | 98 | |
| Range % | 91-105 | |
| 1:20 | Average % | 98 | |
| Range % | 92-110 | |
| 1:40 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human LL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 104 | 92-112 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.420 | 2.468 | 2.444 | 2.246 | | | 5 | 1.875 | 1.895 | 1.885 | 1.687 | | | 2.5 | 1.358 | 1.378 | 1.368 | 1.170 | | | 1.25 | 0.941 | 0.956 | 0.949 | 0.751 | | | 0.625 | 0.552 | 0.589 | 0.571 | 0.373 | | | 0.312 | 0.431 | 0.453 | 0.442 | 0.244 | | | 0.156 | 0.378 | 0.389 | 0.384 | 0.186 | | | 0 | 0.192 | 0.204 | 0.198 | | |
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货期
3-5 working days