产品描述

The human interleukin-36 alpha (IL36A) ELISA kit is suitable for quantitatively determining the human IL36A complex in multiple samples, including serum, plasma, tissue homogenates, or cell culture supernates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human IL36A levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.

IL36A, also called IL1F6, is expressed during embryonic development and is highly abundant in epithelial cells, as well as monocytes, B cells, and T cells. IL36A has been shown to exert strong pro-inflammatory effects in the lungs of mice. Along with its modulation by EGF, IL36A plays an important role in skin barrier function and homeostasis. Ichii et al. indicated an association between intrarenal IL36A overexpression and the progression of tubulointerstitial inflammations and morpho-functional changes of distal tubule (DT) epithelial cells. IL36A may be a novel kidney injury marker useful for evaluating DT damages.

别名

FIL1 ELISA Kit; FIL1 epsilon ELISA Kit; FIL1(EPSILON) ELISA Kit; FIL1E ELISA Kit; IL 1 epsilon ELISA Kit; IL 1F6 ELISA Kit; IL 1H1 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1F6 ELISA Kit; IL1E ELISA Kit; IL1F6 ELISA Kit; IL1F6_HUMAN ELISA Kit; IL1RP2 ELISA Kit; IL36 alpha ELISA Kit; IL36A ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 6 (epsilon) ELISA Kit; Interleukin 1 family member 6 ELISA Kit; Interleukin 36 alpha ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 6 ELISA Kit; MGC129552 ELISA Kit; MGC129553 ELISA Kit; MGC151479 ELISA Kit; MGC151481 ELISA Kit; OTTMUSP00000012798 ELISA Kit; RP23-176J12.4 ELISA Kit

缩写

IL1F6

Uniprot No

Q9UHA7

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		99
		Range %		94-103
1:2		Average %		87
		Range %		80-95
1:4		Average %		95
		Range %		91-99
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human IL1F6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		86-98
EDTA plasma (n=4)		97		92-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 1.966 1.957 1.962 1.861 500 1.432 1.501 1.467 1.366 250 0.950 0.920 0.935 0.834 125 0.680 0.689 0.685 0.584 62.5 0.465 0.502 0.484 0.383 31.2 0.275 0.287 0.281 0.180 15.6 0.182 0.178 0.180 0.079 0 0.098 0.103 0.101

货期

3-5 working days