产品描述

This Human LDHB ELISA Kit was designed for the quantitative measurement of Human LDHB protein in serum, plasma, tissue homogenates, cerebrospinal fluid (CSF), urine.. It is a Sandwich ELISA kit, its detection range is 23.5 mU/mL-1500 mU/mL and the sensitivity is 5.8 mU/mL.

别名

Epididymis secretory protein Li 281 ELISA Kit; HEL S 281 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase B chain ELISA Kit; Lactate Dehydrogenase B ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH-B ELISA Kit; LDH-H ELISA Kit; LDHB ELISA Kit; LDHB_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHH ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-46 ELISA Kit; TRG-5 ELISA Kit; TRG5 ELISA Kit

缩写

LDHB

Uniprot No

P07195

灵敏度

5.8 mU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LDHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		84-93
1:2		Average %		100
		Range %		97-103
1:4		Average %		87
		Range %		84-90
1:8		Average %		102
		Range %		98-106

回收率

The recovery of human LDHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		100-106
EDTA plasma (n=4)		89		85-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected     1500 2.184 2.203 2.194 2.038     750 1.706 1.697 1.702 1.546     375 1.215 1.241 1.228 1.072     187.5 0.757 0.738 0.748 0.592     94 0.442 0.468 0.455 0.299     47 0.319 0.298 0.309 0.153     23.5 0.244 0.231 0.238 0.082     0 0.159 0.153 0.156

货期

3-5 working days