产品描述

This Human JAG1 ELISA Kit was designed for the quantitative measurement of Human JAG1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

AGS ELISA Kit; AHD ELISA Kit; AWS ELISA Kit; CD 339 ELISA Kit; CD339 ELISA Kit; CD339 antigen ELISA Kit; Headturner ELISA Kit; hJ1 ELISA Kit; Htu ELISA Kit; Jag 1 ELISA Kit; Jag1 ELISA Kit; JAG1_HUMAN ELISA Kit; Jagged 1 ELISA Kit; Jagged1 (Alagille syndrome) ELISA Kit; Jagged1 ELISA Kit; JAGL1 ELISA Kit; MGC104644 ELISA Kit; OTTHUMP00000030278 ELISA Kit; Protein jagged-1 ELISA Kit; Ser 1 ELISA Kit; Ser1 ELISA Kit; Serrate 1 ELISA Kit; Slalom ELISA Kit

缩写

JAG1

Uniprot No

P78504

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human JAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		100
		Range %		93-105
1:2		Average %		97
		Range %		90-101
1:4		Average %		98
		Range %		88-102
1:8		Average %		98
		Range %		88-102

回收率

The recovery of human JAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		83-96
EDTA plasma (n=4)		99		89-103

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 10 1.993 1.912 1.953 1.773 5 1.485 1.509 1.497 1.317 2.5 0.975 1.008 0.992 0.812 1.25 0.661 0.645 0.653 0.473 0.625 0.401 0.396 0.399 0.219 0.312 0.305 0.317 0.311 0.131 0.156 0.241 0.250 0.246 0.066 0 0.182 0.178 0.180

货期

3-5 working days