产品描述
This NDM-1 ELISA Kit was designed for the quantitative measurement of NDM-1 protein in serum, plasma, urine samples infected by New Delhi super bacteria. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL- 400 ng/mL and the sensitivity is 3.12 ng/mL.
缩写
NDM-1
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of NDM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
85-103 |
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| 1:2 |
Average % |
92 |
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| Range % |
82-99 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-103 |
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回收率
| The recovery of NDM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-96 |
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| EDTA plasma (n=4) |
96 |
87-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
1.845 |
1.838 |
1.842 |
1.698 |
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| 200 |
1.606 |
1.619 |
1.613 |
1.469 |
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| 100 |
1.337 |
1.340 |
1.339 |
1.195 |
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| 50 |
0.983 |
0.972 |
0.978 |
0.834 |
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| 25 |
0.606 |
0.598 |
0.602 |
0.458 |
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| 12.5 |
0.379 |
0.370 |
0.375 |
0.231 |
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| 6.25 |
0.250 |
0.247 |
0.249 |
0.105 |
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| 0 |
0.145 |
0.143 |
0.144 |
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货期
3-5 working days