产品描述
This Mouse Ox-HDL ELISA Kit was designed for the quantitative measurement of Mouse Ox-HDL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 7.81 pg/mL .
缩写
Ox-HDL
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 95 | |
| Range % | 91-99 | |
| 1:200 | Average % | 94 | |
| Range % | 90-98 | |
| 1:400 | Average % | 98 | |
| Range % | 94-104 | |
| 1:800 | Average % | 84 | |
| Range % | 80-88 | |
回收率
| The recovery of mouse Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 90-97 | |
| EDTA plasma (n=4) | 99 | 95-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | | | | 1000 | 0.097 | 0.096 | 0.097 | | | | 500 | 0.267 | 0.263 | 0.265 | | | | 250 | 0.466 | 0.454 | 0.460 | | | | 125 | 0.691 | 0.687 | 0.689 | | | | 62.5 | 1.080 | 1.091 | 1.086 | | | | 31.25 | 1.391 | 1.412 | 1.402 | | | | 15.6 | 1.504 | 1.606 | 1.555 | | | | 0 | 1.813 | 1.711 | 1.762 | | |
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货期
3-5 working days