产品描述

The human Tau ELISA Kit is engineered for accurate measurement of human Tau levels from samples including serum, plasma, or cerebrospinal fluid (CSF). It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the Tau content in the sample. The color intensity is positively correlated with Tau content in the sample. The Tau concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

Tau, also called MAPT, is the main component of neurofibrillary tangles (NFTs). As a stabilizing microtubule-associated protein (MAP), Tau primarily functions to protect microtubules (MTs) against depolymerization by decreasing the dissociation of tubulin at both MT ends, leading to an elevated growth rate and declined catastrophe frequency. In addition to being a stabilizer of microtubules, Tau is involved in multiple biological processes, including myelination, glucose metabolism, axonal transport, microtubule dynamics, iron homeostasis, neurogenesis, motor function, learning & memory, neuronal excitability, and DNA protection.

别名

AI413597 ELISA Kit; AW045860 ELISA Kit; DDPAC ELISA Kit; FLJ31424 ELISA Kit; FTDP 17 ELISA Kit; G protein beta1/gamma2 subunit interacting factor 1 ELISA Kit; MAPT ELISA Kit; MAPTL ELISA Kit; MGC134287 ELISA Kit; MGC138549 ELISA Kit; MGC156663 ELISA Kit; Microtubule associated protein tau ELISA Kit; Microtubule associated protein tau isoform 4 ELISA Kit; Microtubule-associated protein tau ELISA Kit; MSTD ELISA Kit; Mtapt ELISA Kit; MTBT1 ELISA Kit; MTBT2 ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament tau ELISA Kit; Paired helical filament-tau ELISA Kit; PHF tau ELISA Kit; PHF-tau ELISA Kit; PPND ELISA Kit; PPP1R103 ELISA Kit; Protein phosphatase 1, regulatory subunit 103 ELISA Kit; pTau ELISA Kit; RNPTAU ELISA Kit; TAU ELISA Kit; TAU_HUMAN ELISA Kit; Tauopathy and respiratory failure ELISA Kit; Tauopathy and respiratory failure, included ELISA Kit

缩写

MAPT

Uniprot No

P10636

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-92
1:2		Average %		97
		Range %		92-106
1:4		Average %		98
		Range %		93-105
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human Tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		95-105
EDTA plasma (n=4)		97		91-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.319 2.423 2.371 2.228 500 1.828 1.864 1.846 1.703 250 1.291 1.324 1.308 1.165 125 0.776 0.796 0.786 0.643 62.5 0.464 0.498 0.481 0.338 31.2 0.321 0.341 0.331 0.188 15.6 0.215 0.227 0.221 0.078 0 0.142 0.144 0.143

货期

3-5 working days